Nanodispersion of TiO2 in hypochlorous acid and its antimicrobial effect against oral pathogens

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Published: Dec 14, 2023
Versions:
2023-12-14 (1)
DOI: https://doi.org/10.22201/ceiich.24485691e.2024.33.69813
Keywords:
TiO2 nanoparticles, HOCl, antimicrobial effect, cytotoxic effect, SCAPs

Main Article Content

Juan Rafael Morales-Noriega
Universidad Nacional Autónoma de México, Escuela Nacional de Estudios Superiores Unidad León, León, Guanajuato, México.
https://orcid.org/0009-0007-1707-2851
Christian Andrea López-Ayuso
Universidad Nacional Autónoma de México, Programa de Doctorado en Ciencias Odontológicas, Escuela Nacional de Estudios Superiores Unidad León, Interdisciplinary Research Laboratory (LII), Nanostructures and Biomaterials Area.
https://orcid.org/0000-0003-0534-7823
Laura Susana Acosta-Torres
Universidad Nacional Autónoma de México, Escuela Nacional de Estudios Superiores Unidad León, Interdisciplinary Research Laboratory (LII), Nanostructures and Biomaterials Area.
https://orcid.org/0000-0002-5959-9113
Ravichandran Manisekaran
Universidad Nacional Autónoma de México, Escuela Nacional de Estudios Superiores Unidad León.
https://orcid.org/0000-0002-2934-0717

Abstract

The continuous and inappropriate use of traditionally used antimicrobial agents has caused the rise of multidrug-resistant (MDR) bacterial strains and the mutation of microorganisms in the field of dentistry. Therefore, various nanoparticles have been developed to combat resistant pathogens. Titanium dioxide (TiO2) nanoparticles have been attractive antimicrobial agents because of their chemical stability, non-toxicity, and inexpensive precursors. Therefore, TiO2-based nanodispersions were explored by preparing them with well-known antimicrobial agents, such as hypochlorous acid (HOCl), to enhance the antimicrobial effect. In this study, sol-gel-based TiO2 NPs-HOCl nanodispersions were synthesized and characterized. The antimicrobial effect was assessed by a microdilution assay using S. mutans, S. aureus,  E. faecalis, and C. albicans strains by incubating different concentrations of the nanodispersions. To evaluate the cytotoxic effects, stem cells from the apical papilla (SCAPs) were inoculated and evaluated using the MTT assay. The nanodispersion exhibited an enhanced antimicrobial effect, with almost no cytotoxicity. The HOCl-based nanodispersion exhibited a greater antimicrobial effect and high stability. Thus, it can be used as a promising antimicrobial agent for the treatment of various dental pathogens.

Article Details

How to Cite
Morales-Noriega, J. R., López-Ayuso, C. A., Acosta-Torres, L. S., & Manisekaran, R. (2023). Nanodispersion of TiO2 in hypochlorous acid and its antimicrobial effect against oral pathogens. Mundo Nano. Interdisciplinary Journal on Nanosciences and Nanotechnology, 17(33), 1e-17e. https://doi.org/10.22201/ceiich.24485691e.2024.33.69813
Issue
Vol. 17 No. 33 (2024): 2do semestre / Nanopartículas en la industria, la medicina, la odontología y la producción de hidrógeno
Section
Research articles

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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología por Universidad Nacional Autónoma de México se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
Basada en una obra en http://www.mundonano.unam.mx.

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