Antimicrobial and cytotoxic properties of an orthodontic adhesive added with silver nanoparticles applications

Abstract

The objective of this study was to evaluate the antibacterial effect and cytotoxicity of an orthodontic adhesive with silver nanoparticles (Ag np’s). The antimicrobial activity was determined by a disc diffusion test for Staphylococcus aureus, Escherichia coli, and Enterococcus faecalis at different concentrations of silver nanoparticles in an orthodontic adhesive. As well as cytotoxicity of said adhesive to be incorporated Ag np’s was evaluated, through the mtt method. The results showed that the bacteria under study, S. aureus, E. coli and E. faecalis were susceptible both to the Ag np’s and to the orthodontic mip adhesive added with Ag np’s at 10, 5 and 3 v/v%. The antibacterial effect of silver nanoparticles is dose dependent. The cytotoxicity of the orthodontic mip adhesive itself exhibits a mild to moderate cytotoxicity as well as incorporating the Ag np’s at the concentrations above mentioned. The nanoparticles incorporated in the adhesive for orthodontic use show antibacterial effect as well as maintain a slight cytotoxicity