Evaluating the toxicity of nanomaterials in three-dimensional cellular models
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Abstract
Traditionally, in vitro evaluations to determine the cytotoxic effect of nanomaterials in cell cultures have been carried out in two-dimensional cultures, because the protocols to assess it have been adapted from those used in toxicology. However, the interactions between cells are much more complex than those found in a monolayer arrangement. Thus, this is the main reason to promote the implementation of three-dimensional cell cultures also known as spheroids, to assess the effect of nanomaterials on cell cultures. Abundant supports the idea that spheroids represent a better model for the study of cellular responses, since they emulate more precisely the cellular junctions, communication and physiology that occurs in a tissue within an in vivo model. Herein, we discuss some points about the development of 3D cultures as a new and better methodology for nanotoxicological evaluations.
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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología por Universidad Nacional Autónoma de México se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
Basada en una obra en http://www.mundonano.unam.mx.
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