Nanopartículas de subsalicilato de bismuto por ablación láser: efecto contra bacterias asociadas a infecciones nosocomiales
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Objetivo: evaluar el efecto antibacteriano de nanopartículas de subsalicilato de bismuto (NPs-SSBi) en contra de cuatro bacterias frecuentemente asociadas con infecciones nosocomiales. Métodos: las NPs-SSBi fueron obtenidas en suspensión coloidal por ablación láser de sólidos en líquidos (ALSL). El tamaño, composición y estabilidad de las NPs en suspensión se analizó por microscopía electrónica de transmisión y espectroscopía ultravioleta-visible. El crecimiento planctónico y la formación de biopelícula de dos bacterias Gram-positivas, S. aureus y S. epidermidis, y dos Gram-negativas, E. coli y P. aeruginosa, después de ser expuestas a diferentes concentraciones de NPs-SSBi (1.25 a 90 μg/mL), se evaluó por ensayos de turbidez y XTT, respectivamente. Resultados: se obtuvieron NPs-SSBi cristalinas de forma cuasi-esférica, con un tamaño de 4.5 ± 0.14 nm, las cuales permanecen estables en suspensión coloidal por al menos 21 días. Las NPs-SSBi inhibieron el crecimiento de las cuatro bacterias, el crecimiento planctónico se redujo ≈80-92% a concentraciones por arriba de 40 μL/mL, y la formación de biopelícula ≈73-89% a concentraciones de 80 y 90 μL/mL. Conclusiones: las NPs-SSBi obtenidas por ALSL inhibieron el crecimiento de cuatro importantes bacterias nosocomiales, por lo cual podrían ser utilizadas para el control de infecciones asociadas con la atención a la salud.
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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología, editada por la Universidad Nacional Autónoma de México, se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
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