Solid Calcium Carbonate formation detected by SEM-EDS within the epileptic focus of an in vivo epilepsy model of Wistar rats Formación de cristales de calcio dentro del foco epiléptico de un modelo de epilepsia in vivo

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Publicado: nov. 26, 2024
DOI: https://doi.org/10.22201/ceiich.24485691e.2025.35.69831
Palavras-chave:
calcification, calcium carbonate, epilepsy, Kindling model, SEM

Conteúdo do artigo principal

Tessy López-Goerne
Autonomous Metropolitan University-Xochimilco. Laboratory of Nanotechnology and Nanomedicine, Department of Health Care, Aut, Mexico.
Rafael Valiente
Autonomous Metropolitan University-Xochimilco. Laboratory of Nanotechnology and Nanomedicine, Department of Health Care, Aut, Mexico.
Emilio Chávez
Autonomous Metropolitan University-Xochimilco. Laboratory of Nanotechnology and Nanomedicine, Department of Health Care, Aut, Mexico.
Antonella González-Bondani
Autonomous Metropolitan University-Xochimilco. Laboratory of Nanotechnology and Nanomedicine, Department of Health Care, Aut, Mexico.
Joaquín Manjarrez
National Institute of Neurology and Neurosurgery, Mexico.
Francisco Javier Padilla-Godínez
Autonomous Metropolitan University-Xochimilco. Laboratory of Nanotechnology and Nanomedicine, Department of Health Care, Aut, Mexico. / Western Institute of Technology and Higher Education Department of Mathematics and Physics, Tlaquepaque, Jalisco, Mexico.

Resumo

Epilepsy represents a significant health concern, affecting an estimated 2 million individuals in Mexico, with patients enduring more than 70 seizures daily. In the present study, refractory epilepsy was induced in Wistar rats by repeated stimulation with pentylenetetrazol (PTZ), ranging from one to one hundred stimulations. Hippocampal tissue was analyzed via scanning electron microscopy (SEM) and energy-dispersive X-ray fluorescence (EDS). The formation of solid calcium carbonate (CaCO3) crystals within the epileptic focus where observed. Notably, the concentration of CaCO3 exhibited a direct correlation with the number of stimulations, suggesting a progressive increase over the course of these events. This study puts forth a hypothesis elucidating the mechanism behind CaCO3 crystal formation in response to PTZ-induced seizures. Further research is needed to ascertain whether there exists a correlation between the dysregulation of calcium ions (Ca2+) in epilepsy and the formation of CaCO3. This inquiry opens avenues for deeper understanding and potential advancements in the diagnosis of epilepsy and related neurological disorders.

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López-Goerne, T., Valiente, R., Chávez, E., González-Bondani, A., Manjarrez, J., & Padilla-Godínez, F. J. (2024). Solid Calcium Carbonate formation detected by SEM-EDS within the epileptic focus of an in vivo epilepsy model of Wistar rats: Formación de cristales de calcio dentro del foco epiléptico de un modelo de epilepsia in vivo. Mundo Nano. Revista Interdisciplinaria En Nanociencias Y Nanotecnología, 18(35), e69831. https://doi.org/10.22201/ceiich.24485691e.2025.35.69831
Edição
v. 18 n. 35 (2025): 2do semestre / Nanomateriales como sistemas de liberación de fármacos y biomoléculas / Yareli Rojas Aguirre, Luz María López Marín, José Juan Escobar Chávez, editores invitados.
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Artigos de investigação

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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología, editada por la Universidad Nacional Autónoma de México, se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
Basada en una obra en http://www.mundonano.unam.mx.

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