Biosynthesis of silver nanoparticles with antimicrobial activity by environmental Pseudomonas aeruginosa
Article Sidebar
Main Article Content
Abstract
Silver nanoparticles (NPs-Ag) are of great interest due to the potential applications in different areas. In order to find methodologies that not represent human health risk and also an ecofriendly one, biosynthesis of these nanoparticles has been a viable option. It is known that diverse biomolecules present in microorganisms can function as an oxidative, reductor and/or stabilizer agents, thanks to that silver nanoparticles synthesis could be favored. In this work the main objectives were the biosynthesis, characterization, and evaluation of antibacterial activity of NPs-Ag. Biosynthesis was made using the culture supernatant of Pseudomonas aeruginosa. The NPs-Ag were characterized using UV-Vis spectroscopy, scanning electron microscopy, X‑ray diffraction and the antimicrobial effect was evaluated by dilution assays in Muller Hinton broth and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against Gram-positive and Gram-negative bacteria. A mixture containing 20% of supernatant and 3 mM AgNO3 was the best way to obtain silver nanoparticles. Nanoparticles with mixed morphology were observed. The NPs-Ag showed antibacterial activity over Gram-positive and Gram-negative bacteria with MIC of 1 to 2 µg/ml and MBC of 2 to 4 µg/ml. In conclusion, culture supernatant of P. aeruginosa is a viable pathway for the synthesis of NPs-Ag with antibacterial activity.
Downloads
Article Details
Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología por Universidad Nacional Autónoma de México se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
Basada en una obra en http://www.mundonano.unam.mx.
References
Amendola, V., Bakr, O. M. y Stellacci, F. (2010) A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: Effect of shape, size, structure, and assembly. Plasmonics, 5: 85-97. https://doi.org/10.1007/s11468-009-9120-4.
Durán, N., Durán, M., De Jesús, M. B., Seabra, A. B., Fávaro, W. J. y Nakazato, G. (2016). Silver nanoparticles: a new view on mechanistic aspects on antimicrobial activity. Nanomedicine: Nanotechnology, Biology and Medicine, 12(3): 789-799. https://doi.org/10.1016/j.nano.2015.11.016.
Filloux, A. (2011). Protein secretion systems in Pseudomonas aeruginosa: an essay on diversity, evolution, and function. Frontiers in Microbiology, 2, 155.x. https://doi.org/10.3389/fmicb.2011.00155.
Flores-Pantoja, L. E., Briseño-Silva, E, Loeza-Lara, P. D. y Jiménez-Mejía, R. (2022). Actividad antifúngica y características de promoción de crecimiento vegetal de Pseudomonas aeruginosa y Enterobacter sp. degradadoras de hidrocarburos aisladas de suelo contaminado. Acta Biológica Colombiana, 27(3). https://doi.org/10.15446/abc.v27n3.92758.
Franci, G., Falanga, A., Galdiero, S., Palomba, L., Rai, M., Morelli, G. y Galdiero, M. (2015). Silver nanoparticles as potential antibacterial agents. Molecules, 20(5): 8856-8874. https://doi.org/10.3390/molecules20058856.
Gul, S., Ismail, M., Khan, M. I., Khan, S. B., Asiri, A. M., Rahman, I. U., Kahn, M. A. y Kamboh, M. A. (2016). Novel synthesis of silver nanoparticles using melon aqueous extract and evaluation of their feeding deterrent activity against housefly Musca domestica. Asian Pacific Journal of Tropical Disease, 6(4): 311-316. https://doi.org/10.1016/S2222-1808(15)61036-2.
Huq, M. (2020). Green synthesis of silver nanoparticles using Pseudoduganella eburnea MAHUQ-39 and their antimicrobial mechanisms investigation against drug resistant human pathogens. International Journal of Molecular Sciences, 21(4): 1510. https://doi.org/10.3390/ijms21041510.
Huq, M. A., Ashrafudoulla, M., Rahman, M. M., Balusamy, S. R. y Akter, S. (2022). Green synthesis and potential antibacterial applications of bioactive silver nanoparticles: a review. Polymers, 14(4), 742. https://doi.org/10.3390/polym14040742.
Iravani, S. (2014). Bacteria in nanoparticle synthesis: current status and future prospects. International Scholarly Research Notices, 2014. https://doi.org/10.1155/2014/359316.
Khan, I., Saeed, K. y Khan, I. (2017). Nanoparticles: properties, applications and toxicities. Arabian Journal of Chemistry, 12: 908-931. https://doi.org/10.1016/j.arabjc.2017.05.011.
Kim, S. W., Chung, H. E., Kwon, J. H., Yoon, H. G. y Kim, W. (2010) Facile synthesis of silver chloride nanocubes and their derivatives. Bulletin of the Korean Chemical Society; 31: 2918-22. https://doi.org/10.5012/bkcs.2010.31.10.2918.
Kumar, C. G. y Mamidyala, S. K. (2011). Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa. Colloids and Surfaces B:Biointerfaces, 84(2): 462-466. https://doi.org/10.1016/j.colsurfb.2011.01.042.
Kumar, C. G., Mamidyala, S. K., Das, B., Sridhar, B., Devi, G. S. y Karuna, M. S. (2010). Synthesis of biosurfactant-based silver nanoparticles with purified rhamnolipids isolated from Pseudomonas aeruginosa BS-161R. Journal of Microbiology and Biotechnology, 20(7): 1061-8. https://doi.org/10.4014/jmb.1001.01018.
Madani, M., Hosny, S., Alshangiti, D. M., Nady, N., Alkhursani, S. A., Alkhaldi, H., Al-Gahtany, S. A., Ghobashy, M. M. y Gaber, G. A. (2022). Green synthesis of nanoparticles for varied applications: Green renewable resources and energyefficient synthetic routes. Nanotechnology Reviews, 11(1): 731-759. https://doi.org/10.1515/ntrev-2022-0034.
Naganthran, A., Verasoundarapandian, G., Khalid, F. E., Masarudin, M. J., Zulkharnain, A., Nawawi, N. M., Karim, M., Che Abdullah, C. A. y Ahmad, S. A. (2022). Synthesis, characterization and biomedical application of silver nanoparticles. Materials, 15(2): 427. https://doi.org/10.3390/ma15020427.
Pantidos, N. y Horsfall, L. E. (2014). Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. Journal of Nanomedicine and Nanotechnology, 5(5): 1. http://dx.doi.org/10.4172/2157-7439.1000233.
Rabiee, N., Ahmadi, S., Akhavan, O. y Luque, R. (2022). Silver and gold nanoparticles for antimicrobial purposes against multi-drug resistance bacteria. Materials, 15(5): 1799. https://doi.org/10.3390/ma15051799.
Rai, M. K., Deshmukh, S. D., Ingle, A. P. y Gade, A. K. (2012). Silver nanoparticles: the powerful nanoweapon against multidrug‐resistant bacteria. Journal of Applied Microbiology, 112(5): 841-852. https://doi.org/10.1111/j.1365-2672.2012.05253.x.
Restrepo, C. V. y Villa, C. C. (2021). Synthesis of silver nanoparticles, influence of capping agents and dependence on size and shape: A review. Environmental Nanotechnology, Monitoring & Management, 15, 100428. https://doi.org/10.1016/j.enmm.2021.100428.
Rudramurthy, G. R., Swamy, M. K., Sinniah, U. R. y Ghasemzadeh, A. (2016). Nanoparticles: alternatives against drug-resistant pathogenic microbes. Molecules, 21(7): 836. https://doi.org/10.3390/molecules21070836.
Sharma, V. K., Yngard, R. A. y Lin, Y. (2009). Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science, 145(1-2): 83-96. https://doi.org/10.1016/j.cis.2008.09.002.
Siddiqi, K. S., Husen, A. y Rao, R. A. (2018). A review on biosynthesis of silver nanoparticles and their biocidal properties. Journal of Nanobiotechnology, 16(1): 14. https://doi.org/10.1186/s12951-018-0334-5.
Singh, H., Du, J., Singh, P. y Yi, T.H. (2018). Extracellular synthesis of silver nanoparticles by Pseudomonas sp. THG-LS1.4 and their antimicrobial application. Journal of Pharmaceutical Analysis, 8: 258-264. https://doi.org/10.1016/j.jpha.2018.04.004.
Singh, P., Kim, Y. J., Zhang, D. y Yang, D. C. (2016). Biological synthesis of nanoparticles from plants and microorganisms. Trends in Biotechnology, 34(7): 588-599. https://doi.org/10.1016/j.tibtech.2016.02.006.
Tada, S., Watanabe, F., Kiyota, K., Shimoda, N., Hayashi, R., Takahashi, M., Nariyuki, A., Igarashi, A., Satokawa, S. (2017). Ag addition to CuO-ZrO2 catalysts promotes methanol synthesis via CO2 hydrogenation. Journal of Catalysis. 351 (2017): 107-118. http://dx.doi.org/10.1016/j.jcat.2017.04.021.
Thakkar, K. N., Mhatre, S. S. y Parikh, R. Y. (2010). Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 6(2): 257-262. https://doi.org/10.1016/j.nano.2009.07.002.
Wang, L., Hu, C. y Shao, L. (2017). The antimicrobial activity of nanoparticles: present situation and prospects for the future. International Journal of Nanomedicine, 12: 1227. https://doi.org/10.2147/IJN.S121956.
Yin, I. X., Zhang, J., Zhao, I. S., Mei, M. L., Li, Q. y Chu, C. H. (2020). The antibacterial mechanism of silver nanoparticles and its application in dentistry. International Journal of Nanomedicine, 15, 2555-2562. https://doi.org/10.2147/IJN.S246764.