Desarrollo y evaluación de un sensor portátil para la detección de Hg2+, a través de la síntesis verde de AuNP utilizando extracto acuoso de Sargassum spp.
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El presente trabajo tiene la finalidad de dar a conocer el desarrollo de un prototipo de sensor para la medición de mercurio (Hg2+) en solución acuosa sobre una plataforma electroquímica basada en electrodos serigrafiados de nanotubos de carbonos (CNT-SPE) en conjunto con nanopartículas de oro (NPs-Au) realizadas a través de síntesis verde, utilizando extracto acuoso de Sargassum spp. Para esto, se realizó la caracterización de las NPs-Au por espectroscopía ultravioleta-visible (UV-Vis), infrarrojo (TF-IR), difracción de rayos X (DRX) y microscopía electrónica de barrido (SEM); obteniendo nanopartículas entre 80-100 nm las cuales poseen un recubrimiento orgánico que les confiere una sinergia al momento de ser conjugadas con CNT-SPE oxidadas/activadas. Posteriormente, las NPs-Au se utilizaron para modificar el CNT-SPE a través del método drop-casting y así ensamblar un novedoso sensor electroquímico para la medición de Hg2+, cuya validación y habilidad para medir fue demostrada utilizando voltametría diferencial de pulso (DPV). Se obtuvieron límites de detección de 2.38 uM y cuantificación de 3 uM. Los resultados demuestran que la plataforma desarrollada es una alternativa rápida, eficiente y portátil para la medición de Hg2+.
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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología, editada por la Universidad Nacional Autónoma de México, se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
Basada en una obra en http://www.mundonano.unam.mx.
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Abdoli M, Arkan E, Shekarbeygi Z, Khaledian S. Green synthesis of gold nanoparticles using Centaurea behen leaf aqueous extract and investigating their antioxidant and cytotoxic effects on acute leukemia cancer cell line (THP-1). Inorganic Chemistry Communications. 2021;129:108649-. DOI: https://doi.org/10.1016/j.inoche.2021.108649
Al-Radadi NS. Facile one-step green synthesis of gold nanoparticles (AuNp) using licorice root extract: Antimicrobial and anticancer study against HepG2 cell line. Arabian Journal of Chemistry. 2021;14(2):102956-. DOI: https://doi.org/10.1016/j.arabjc.2020.102956
Asnag GM, Oraby AH, Abdelghany AM. Green synthesis of gold nanoparticles and its effect on the optical, thermal and electrical properties of carboxymethyl cellulose. Composites Part B: Engineering. 2019;172:436-4. DOI: https://doi.org/10.1016/j.compositesb.2019.05.044
Bernhoft RA. Mercury toxicity and treatment: a review of the literature. Journal of Environmental and Public Health. 2012;2012:460508-. DOI: https://doi.org/10.1155/2012/460508
Camacho-de la Cruz AA, Espinosa-Reyes G, Rebolloso-Hernández CA, Carrizales-Yáñez L, Ilizaliturri-Hernández CA, Reyes-Arreguín LE, Díaz-Barriga F. Environmental Monitoring and Assessment. 2021.
Chen X- yue, Xue Z, Ji J, Wang D, Shi G, Zhao L- gong, Feng S. Hedysarum polysaccharides mediated green synthesis of gold nanoparticles and study of its characteristic, analytical merit, catalytic activity. Materials Research Bulletin. 2020;133:111070-. DOI: https://doi.org/10.1016/j.materresbull.2020.111070
Cho I-H, Kim DH, Park S. Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis. Biomaterials Research. 2020;24(1):6-. DOI: https://doi.org/10.1186/s40824-019-0181-y
Ebdon L, Foulkes ME, Le Roux S, Muñoz-Olivas R. Cold vapour atomic fluorescence spectrometry and gas chromatography-pyrolysis-atomic fluorescence spectrometry for routine determination of total and organometallic mercury in food samples. Analyst. 2002;127(8):1108-14. DOI: https://doi.org/10.1039/B202927H
Frena M, Campestrini I, Braga OC de, Spinelli A. In situ bismuth-film electrode for square-wave anodic stripping voltammetric determination of tin in biodiesel. Electrochimica Acta. 2011;56(12):4678-84. DOI: https://doi.org/10.1016/j.electacta.2011.02.111
González-Reyna MA, España-Sánchez BL, Molina GA, López-Miranda JL, Mendoza-Cruz R, Esparza R, Estevez M. Carbon dots synthesized from Cinchona pubescens Vahl. An efficient antibacterial nanomaterial and bacterial detector. ChemistrySelect. 2022;7(17). DOI: https://doi.org/10.1002/slct.202104530
Guo W, Yuan J, Wang E. Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion. Chemical Communications. 2009;(23):3395-7. DOI: https://doi.org/10.1039/b821518a
Hammami I, Alabdallah NM, Al jomaa A, kamoun M. Gold nanoparticles: synthesis properties and applications. Journal of King Saud University - Science. 2021;33(7):101560-. DOI: https://doi.org/10.1016/j.jksus.2021.101560
Karthika A, Ramasamy Raja V, Karuppasamy P, Suganthi A, Rajarajan M. Electrochemical behaviour and voltammetric determination of mercury (II) ion in cupric oxide/poly vinyl alcohol nanocomposite modified glassy carbon electrode. Microchemical Journal. 2019;145:737-44. DOI: https://doi.org/10.1016/j.microc.2018.11.030
Lee JH, Kim HJ, Jee Y, Jeon Y-J, Kim HJ. Antioxidant potential of Sargassum horneri extract against urban particulate matter-induced oxidation. Food Science and Biotechnology. 2020;29(6):855-6. DOI: https://doi.org/10.1007/s10068-019-00729-y
Lim JW, Kim T-Y, Woo M-A. Trends in sensor development toward next-generation point-of-care testing for mercury. Biosensors and Bioelectronics. 2021;183:113228-. DOI: https://doi.org/10.1016/j.bios.2021.113228
Muniyappan N, Pandeeswaran M, Amalraj A. Green synthesis of gold nanoparticles using Curcuma pseudomontana isolated curcumin: its characterization, antimicrobial, antioxidant and anti-inflammatory activities. Environmental Chemistry and Ecotoxicology. 2021;3:117-24. DOI: https://doi.org/10.1016/j.enceco.2021.01.002
Polo LK, Chow F. Variation of antioxidant capacity and antiviral activity of the brown seaweed Sargassum filipendula (Fucales, Ochrophyta) under UV radiation treatments. Applied Phycology. 2022;3(1):260-73. DOI: https://doi.org/10.1080/26388081.2022.2030653
Poulin J, Gibb H, Prüss-Üstïm A. Mercurio: Evaluación de la carga de morbilidad ambiental a nivel nacional y local. 2013;.
Prasedya ES, Martyasari NWR, Abidin AS, Ilhami BTK, Padmi H, Widyastuti S, Sunarwidhi AL, Sunarpi H. Antioxidant activity of brown macroalgae Sargassum ethanol extract from Lombok coast, Indonesia. IOP Conference Series: Earth and Environmental Science. 2021;712(1):012038-. DOI: https://doi.org/10.1088/1755-1315/712/1/012038
Satpathy S, Patra A, Ahirwar B, Hussain MD. Process optimization for green synthesis of gold nanoparticles mediated by extract of Hygrophila spinosa T. Anders and their biological applications. Physica E: Low-dimensional Systems and Nanostructures. 2020;121:113830-. DOI: https://doi.org/10.1016/j.physe.2019.113830
Singh AK, Tiwari R, Singh VK, Singh P, Khadim SR, Singh U, , Srivastava V, Hasan SH, Asthana RK. Green synthesis of gold nanoparticles from Dunaliella salina, its characterization and in vitro anticancer activity on breast cancer cell line. Journal of Drug Delivery Science and Technology. 2019;51:164-76. DOI: https://doi.org/10.1016/j.jddst.2019.02.023
Thakur NS, Mandal N, Banerjee UC. Esterase-mediated highly fluorescent gold nanoclusters and their use in ultrasensitive detection of mercury: synthetic and mechanistic aspects. ACS Omega. 2018;3(12):1855318562-. DOI: https://doi.org/10.1021/acsomega.8b02505
Mineral commodity summaries 2020. Reston, VA: U. S. Geological Survey; 2020.
Usman AI, Aziz AA, Noqta OA. Green sonochemical synthesis of gold nanoparticles using palm oil leaves extracts. Materials Today: Proceedings. 2019;7:803-7. DOI: https://doi.org/10.1016/j.matpr.2018.12.078
Yeh Y-C, Creran B, Vincent MR. Gold nanoparticles: preparation, properties, and applications in bionanotechnology. Nanoscale. 2012;4(6):1871-80. DOI: https://doi.org/10.1039/C1NR11188D