Introduction
The world of consumer wearable electronic devices drives energy storage and augmentation research with low costs and environmentally friendly. The greater use of renewable energy will imply new technology systems and distribution, transmission, and storage systems management. Furthermore, it is necessary to advance in battery technology, electric double layer capacitors (EDLC), and for this, it is essential to advance the technology of energy storage, like batteries and electrical double-layer capacitors (EDLC’s) to make them economically viable in wide forms of application (CGEE, 2008). Prospective studies indicate that in the next three decades, renewable energy sources will have an expansion of approximately four times related to the installed capacity, implying a 50% reduction of CO2 emissions concerning the current volume. In this scenario, electricity will represent up to 50% of the world’s energy, so it is imperative to build new solutions for energy storage that are not yet available and can cope with the predicted demands (Chu, Majumdar, 2012) and with the environmental demands. The EDLC are complementary energy storage devices to batteries, occupying a niche position with high power densities (Hu et al., 2009; Lu, 2013).
New and innovative materials, such as multi-walled carbon nanotubes (MWCNT)) are used in energy-stored devices to increase energy density and lifespan (Vicentini et al., 2019). Nanomaterials, especially carbon nanotubes (CNT), are attractive in this context (Du, Pan, 2006). The nanomaterials have significant relevance due to their electrical, mechanical, and thermal properties that differ with the size of the particles that compose them (Buzea et al., 2007; Ramrakhiani, 2012).
The MWCNT constitute one of the most promising nanotechnology classes (Petersen, Henry, 2012). They are extensions of sp2 carbon atoms arranged on fused benzene rings. Their structures give exceptional material properties, having applications in composite materials, sensors, and energy storage cells, in addition to various environmental applications (Dillon et al., 1997; Snow et al., 2005; Dalton et al., 2003; Mauter, Elimelech, 2008).
Although they demonstrate great applicability, products containing nanomaterials can generate manufacturing waste and other harmful environmental factors. Due to the incorporation of nanoparticles (NP) into commercial products, on a large scale, its incorporation into environmental matrices (water, air, and earth) can occur in any stage of the life cycle of products (Lovern et al., 2007; Keller et al. 2013; Mitrano et al., 2015).
Although the MWCNT in this study are obtained by an environmentally friendly process (Vicentini et al., 2018) using manly Camphor (C10H16O) and ethanol (C2H5OH), these devices, once in the environment due to improper disposal, will be decomposed. Their constituents will have their final destination in water bodies (Ren et al., 2015; Zhao et al., 2010; Gottschalk et al., 2009; Cornelis et al., 2014), where they can be consumed by primary species and undergo magnification in the food chain (Pakarinen et al., 2013). More and more evidence has been presented which shows an association of CNT with potentially dangerous effects on cells, tissues, and organisms (Van der Zande et al., 2010; Braun et al., 2008; Johnston et al., 2010; Zhao et al., 2012; Zhao et al., 2021). However, due to different experimental conditions, including the properties of CNT, the chemistry of the test medium, and species of organisms, the results of CNT toxicity studies are often contradictory, and there is no consensus regarding their potential impacts on these ecosystems, thus the environmental safety of these devices must be guaranteed. Soil is an essential environmental sink of CNT (Gottschalk et al., 2009, Cornelis et al., 2014) through its application of sewage sludge or by the disposal of e-waste (Tourinho et al., 2012). Unfortunately, our understanding of the possible adverse effects of these NP on the environment and human health is still lagging behind their rapid incorporation into commercial products (Magdolenova et al., 2014; Kim et al., 2019).
Several studies have shown that an important mechanism among those involved in NP toxicity refers to the induction of oxidative stress (Valant et al., 2012; Hu et al., 2015; Chen et al., 2016; Waissi et al., 2017; Irazusta et al., 2018). Inflammation, immunotoxicity, and genotoxicity have also been postulated as underlying mechanisms of NP toxicity (Magdolenova et al., 2014), and the mechanism behind these toxic effects may be due to its ability to bind proteins.
The proliferation of nanotechnology-based products in recent decades has spawned a new environment-related sub-discipline, the nanotoxicology (Kahru, Dubourguier, 2010; Boyes et al., 2017). This science also considers the transfer of these nanomaterials in the food chain, causing the accumulation of non-biodegradable pollutants and may affect the bioavailability of other toxicants by facilitating their transport (Mahapatra et al., 2015; Neal, 2008). The unique properties of these NP, so widely described, such as their size, varied shape, and high surface area (Federici et al., 2007; Paschoalino et al., 2010) that make them so attractive, may also potentially be responsible for harmful effects to living organisms, as reported in toxicological studies in several species, such as algae, fish, rodents and human cells (Tong et al., 2007; Oberdörster, 2005; Gomes et al., 2018, Irazusta et al., 2018, Zhao et al., 2021).
Therefore, this work evaluated the potential impacts of MWCNT removed from EDLC using two bioindicator models, unicellular green algae of the species Raphidoceles subcapitata and oligochaetes of the species Eisenia andrei, aiming to contribute to the productive sector regarding the life cycle of new materials, especially related to electronic waste.
Materials and methods
Microporous carbon electrodes with high defective carbon nanotubes (HD-CNT)
Electric double layer capacitors (EDLC) were composed of aluminum foil as a current collector and a microporous carbon active material so that the microporous carbon adheres well to the steel sheet. In electroplating, the engraved aluminum foil is used as the cathode and the nickel rod as the anode. Electrodeposition is performed at a current density of 48 mA. cm-2 at 60 ºC for 1 minute. The aluminum foil is dried under ambient conditions and finally cut into 1 cm diameter circles (area 1.93 cm2) suitable for assembly. A stainless steel spring and separator were placed at the base of the cell as current collectors. The manufacture of EDLC and characterizations by Raman spectroscopy were performed at the Carbon Sci-Tech laboratory, Unicamp, SP, Brazil. Figure 1 shows a schematic of the composition of the EDLC.
Figure 1
Electric double layer capacitors (EDLC). In (a) top view of the coin cell and in (b) schematic diagram of the assembly.
Source: Adapted from Vicentini et al. (2018).
Multi-walled carbon nanotubes (MWCNT) were removed from the EDLC and solubilized in distilled water at concentrations of 0.1; 1.0; 10.0 and 100 mg.L-1. The solutions were sonicated in an ultrasound device for 40 minutes to improve the CNT dissolution.
Bioassay with R. subcapitata
From a seven-day culture of algae of the species Raphidoceles subcapitata, an inoculum with 2.56 x 105 cels.mL-1 was performed. In a volume of 2.5 mL of each concentration of MWCNT, or of buffered water (1.5 mM NaHCO3), 100 µL of the algal inoculum was added. The samples were kept for 72 hours under agitation and constant lighting. After this time, the algal biomass was determined by counting in a Neubauer chamber (Environment Canada, 1992 EPS1/RM/25). The samples were prepared in triplicate and the averages of the counts are compared by Student’s “t” test, assuming 95% confidence interval in the Prisma 5,0 program.
Bioassay with Eisenia Andrei
Acute toxicity
The test organism matrices were kindly provided by the Environmental Sanitation Laboratory (LABSAN) of the Civil Engineering Faculty of the State University of Campinas, SP-Brazil. The organisms were placed in substrate as recommended in the standard (ABNT NBR 15537:2014) for a period of 15 to 30 days for acclimatization. The organisms after this time were classified as large (from 2 cm in length) and small (below 2 cm in length). Ninety large earthworms were used. A mixture was prepared containing 700 g of sand, 200 g of earth and 100 g of coconut fiber powder, where the worms were placed for conditioning for 24 hours. After acclimatization, the earthworms were divided into 10 pots (500 g) with the same soil composition, with MWCNT at concentrations of 0.1-1.0-10.0 and 100 mg. Kg-1, each experimental group was done in triplicate. The organisms were evaluated for mortality and body mass variation after 7 and 14 days of exposure.
Comet test
The comet test evaluates genomic lesions that result in non-reversible mutations in DNA (Singh et al., 1988). The assay consists of cell lysis, DNA relaxation and electrophoresis. After being stained, it is possible to analyze the running pattern of the DNA fragments that form a “tail”. The size of the tail allows classifying the level of damage caused by the analyzed compound or environmental sample.
The assay followed the protocol of Singh et al. (1988). To perform the comet assay, 3 organisms from each treatment were euthanized by exposure to cold (4 hours at 4-5 ºC). For cell extraction, the organisms were immersed in 10% ethanol for 1 hour. It was then centrifuged at 3000 RPM for 10 minutes, the supernatant was removed and the pellet was resuspended with 500 µL of Phosphate Saline Buffer (0.137mM NaCl, 27mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4). Cell viability assessment was performed with Trypan Blue (0.4% in PBS). For the comet, samples were prepared by mixing 80 µL of the cell suspension with 120 µL of Low Melting agarose (Sigma-Aldrich A9414) and applied on slides previously prepared with a layer of 1% agarose (Sigma-Aldich A9539) and, then they were covered with coverslips. The slides remained in the refrigerator for 15 minutes and then the coverslips were gently removed, taking care to not remove the material. The hydrolysis was then carried out with a lysis solution (25mM NaCl, 100mM EDTA, TRIS10mM, 1% N-lauroyl-sarcosine, 0.1% TRITON-X100, 10% Dimethylsulfoxide), for 60 minutes in the refrigerator, followed by washing for remove excess solution. The slides were immersed in electrophoresis buffer (1.5 mM EDTA, 30 mM NaOH) in an ice bath for 20 minutes, followed by running for 30 minutes (300 mA and 26 V). After washing and neutralizing with 0.4M TRIS buffer, the slides were dried in an oven at 37 ºC for 2 hours and fixed with a fixative solution (0.92M Trichloroacetic acid, 0.043M ZnSO4.7H2O, 5% Glycerol), and dried in an oven at 37 ºC. After rehydration, they were stained with DAPI (4’,6-diamidino-2-phenylindole) for 30 minutes and observed under a fluorescence microscope.
Cytotoxicity
A drop of the sediment obtained in the previous step was deposited and spread on a slide. After complete drying of the smear, staining with Leishman’s stain was performed. After drying the cells were observed for cytoplasmic or nuclear changes, as well as the presence of micronuclei in the optical microscopy.
Results and discussion
Carbon-based nanomaterials, one of the attractive nanomaterials with various shapes, covering fullerenes, single- and multi-walled carbon nanotubes, carbon nanoparticles, graphene, among others, are at the forefront of the rapidly developing field of nanotechnology (Zhang et al., 2013). In addition to the influence of charge transfer, the interaction of carbon nanomaterials and cellular compounds, like proteins are also related to Van der Waals force and Coulomb force (Hou et al., 2015).
The Raman scattering spectroscopy technique has been widely used in the characterization of carbonaceous materials. With microfocusing resources, the investigations are very precise, identifying the different crystalline and amorphous forms that can compose the samples. According to their possible applications, a precise characterization of carbonaceous materials is necessary, preferably by non-destructive methods, with analyzes not only regarding their heterogeneity, but also regarding their structural form (Lobo et al., 2012).
In this study, carbon nanotubes were characterized by Raman spectroscopy (Figure 2A), scanning electron microscopy (SEM) (Figure 2B), transmission electronic microscopy (TEM) (Figure 2C). The peak observed at 1300 cm-1, referring to the D band, and the peak at 1500 cm-1, referring to the G band, are characteristic of sp2 carbon bonds. The peak at 2620 cm-1 represents the D+G sum, confirming the carbon nanostructured nature of the material studied. The nano dimentions of the material was confirmed, as can be seen by SEM and TEM characterizations.
Figure 2
(A) Raman spectrum of MWCNT, ƛ 485nm, showing the D, G and D+G bands, (B) SEM and (C) TEM of the multi-walled carbon nanotubes electrodes supported onto the Ni x Al current collector.
Source: Adapted from Vicentini et al. (2018).
Algae are primary producers in the aquatic environment and, therefore, are an important tool in monitoring studies (Saxena et al., 2020). These organisms present a rapid physiological response, which allows the detection of deleterious effects caused by toxic compounds in the short term (Nogueira et al., 2015; Lu et al., 2021). Our results showed that the MWCNT removed from the EDLC inhibited the growth of algal biomass, in a concentration-dependent manner at concentrations higher than 10mg.L-1 (Figure 3), the EC50 was 0.73 mg.L-1.
Figure 3
Raphidoceles subcapitata toxicity bioassay; algal biomass growth inhibition test (p < 0.05).
Source: Author’s elaboration.
Raman spectroscopy also showed spctra confiming an interaction between CNT and algae cells. In Figure 4A it is shown the spectrum corresponding to algae in the culture medium with and without CNT at a concentration of 10mg.L-1. The Raman spectrum characteristic of the freshwater algae Raphidocelis subcapitata shows bands between 1000 and 2000 cm-1 corresponding to the cellular structure of plants (Figure 4A), as the band 1555.27 cm-1 corresponds to the clear carotenoid (Parab, Tomar, 2012; Reynolds, Giltrap, Chambers, 2021), the band at 1337.96 cm-1 corresponds to the elongation mode of the C-C bond characteristic of type a chlorophyll biomolecules (Chl a) (Parab, Tomar, 2012). In addition there is the band 1019.51 cm-1, which corresponds to the common carotenoid of algae, this biomolecule exerts the protective action of chlorophyll (Gall, Pascal, Robert, 2015; Parab, Tomar, 2012; Reynolds et al., 2021). The Raman spectrum for the algae culture exposed to CNT is shown in Figure 4B, where it is observed distint characteristics in the spectrum of the algae cells , in the spectrum it is observed a decrease in the intensity of the bands that are structural features of algae. However, it is possible to observe the D band around 1320 cm-1, the G and D bands around 1580 and 1610 cm-1 , as well as the D+G band around 2620 cm-1.
Figure 4
Raman spectrum referring to the algae Raphidocelis subcapitata before and after exposure to CNT.
Source: Author’s elaboration.
These characteristic bands of the CNT also showed a reduction in intensity and an increase in the width of the bands, indicating component overlaps. Thus, it is possible to affirm that the structural modification of both algae and CNT occurred, due to their physical interaction (Zhang et al., 2015).
In A, the bands referring to the algal are observed; in B. the bands corresponding to algae exposed to MWCNT are presented, where the D band is observed around 1320 cm-1, the G and D’ bands around 1580 and 1610 cm-1 and also the D+ band. G around 2620 cm-1. The characteristic bands of the CNT showed a reduction in intensity and an increase in the width of the bands, indicating the overlapping of components.
Toxicity analyzes have already demonstrated inhibition of algal growth after exposure to CNT (Patel et al., 2019). The reduction in growth would be due to physical interactions or agglomeration of nanoparticles with the cell surface. The opacity of CNT and the agglomeration next to the algal cells cause a “shading” that could explain the inhibition of their growth (Wick et al., 2007; Schwab et al., 2011), although for some authors, this effect is insignificant (Kwok et al., 2010; Long et all., 2012), attributing the inhibition to the physical interaction with the cell surface (Saxena et al., 2020), destruction of the plasma membrane (Hu et al., 2015) and internalization (Gomes et al., 2018; Irazusta et al., 2021), all of them also leading to metabolic changes resulting from the production of reactive oxygen species (ROS) and oxidative stress (Long et al., 2012; Nogueira et al., 2015). In fact, it was previously stated that the interaction of CNT with cellular proteins may be a common path to their biological effects (Wang et al., 2019).
Algae Pseudokirchneriella subcapitata (R. subcapitata) and Chlorella vulgaris exposed to SWCNT showed decreased growth rate, with EC50 of 29.99 and 30.96 mg.L-1, respectively (Sohn et al., 2015), cellular damage and oxidative stress (Hu et al., 2015). In another study, the inhibition concentration (IC25) of 1.04 mg.L-1 was determined in 72 h for P. subcapitata (Blaise et al., 2008). In our study the EC50 was 0.73mg.L-1, it is observerd a great variation in these values, probably due CNT characteristics.
Thus, the algae Pseudokirchneriella subcapitata, renamed Raphidocelles subcapitata constitute one of the most sensitive organisms of the aquatic biota in relation to the toxicity of these nanoparticles and are adversely affected (Blaise et al., 2008). Reduced graphene oxide (rGO), another carbonaceous nanoparticle, also inhibited the growth of R. subcapitata algae and was internalized by algal cells, causing metabolic and structural changes related to structural proteins (Irazusta et al., 2021). Carbon nanomaterial agglomerated and/or internalized by cells not only reduces the availability of light, but also interrupts the provision of sufficient nutrition. This restricted supply leads to inhibition of algal growth and viability. Exposure also results in the generation of stress within the algal cells, production of reactive oxygen species and elevation of stress enzymes, revealing an adverse condition within the algal cells. Despite this, contrasting results of increased algal growth have also been reported recently (Zhang et al., 2018a, b, c). This is a general impact, but not the same for all algae species.
Earthworms are widely studied among soil invertebrates, as they play a key role in most terrestrial ecosystems and represent a significant portion of soil macrofauna (Courtois et al., 2021). They are also regarded as “soil engineers” involved in maintaining soil structure and fertility (Carbonell et al., 2009), are also widely used as bioindicator organisms in toxicity in studies to measure the effects of soil contaminants such as pesticides, heavy metals, pathogens, microplastics and nanomaterials (Gu et al., 2017; Xiao et al., 2020; Sun et al., 2021; Forbes et al., 2021; Bourdineaud et al., 2021). Increased production and environmental release of multi-walled carbon nanotubes (MWCNTs) increase soil exposure and potential risk to earthworms. Besides, CNT toxicity to earthworms remains unclear, with some studies identifying negative effects and others negligible effects.
In this study, after 14 days of exposure of the organisms, there was 5% (1/20) mortality at concentrations of 1.0 and 10 mg. Kg-1 and there was no significant mass variation in relation to the control, except for 1.0 mg. Kg-1 concentration (Figure 5).
Figure 5
Body mass variation (g) after 14 days of exposure of Eisenia andrei earthworms to MWCNT.
Source: Author’s elaboration.
The comet assay did not detect significant damage at any of the concentrations (Figure 6), but a subletal effect was demonstrated by cytotoxicity analysis, by identify intense cytoplasmic vacuolization (Figure 7), witch is a indicative of a metabolic diversion for detoxification. Sublethal toxic effects have also been reported, both in metabolism, due to the increase in ROS production, and in histopathological alterations, with malformations in E. andrei oligochaetes exposed to PdCu/MWCNand PdCu/MWCNT at a concentration of 2000 mg.k-1 (Köktürk et al., 2021). Recent study suggests that toxicity of MWCNTs to earthworms is associated with reduced detoxification capacity, excessive oxidative stress, and disturbance of multiple metabolic pathway, corroboranting with the probably interaction with cellular proteins (Wang et al., 2019). These effects can be amplified by the characteristics of bioaccumulation of MWCNT in earthworms, as demonstrated by Petersen et al. (2010) and Li et al. (2013).
Figure 6
Quantification of DNA damage by the Comet assay, considering damage levels from 0-4. “D” means damage level, rang 0 to 4.
Source: Author’s elaboration.
Conclusion
As already mentioned, nanomaterials have a wide variety of applications and due to this broad distribution, the understanding of their pottential contaminant effects on the environment is crucial. The nanotechnology advances in recent decades has enabled a great development in the production of synthetic nanoparticles, whose wide application also guarantees the release of relevant amounts of them into the environment from industrial areas, water treatment plants, etc. (Nowack et al., 2012) and raises concerns regarding the safety of these nanoparticles in relation to their toxicity and the potential risks resulting from their presence in the ecosystems (Unrine et al., 2010; Cornelis et al., 2014). Furthermore, MWCNT have been shown to tend to adsorb a wide variety of toxic substances, which can increase the toxicity of chemicals in organisms (Braun et al., 2008). As demonstrated in this work, MWCNT showed aquatic toxicity in algae of the species R. subcapitata. In the terrestrial environment, no acute toxicity was observed, such as mortality in the bioindicator E. andrei, nor chronic effects, such as genotoxicity, but a sublethal effect, such as cellular suffering, was demonstrated by the intense cytoplasmic vacuolization, indicating a cellular response to stress, diverting the energy necesserary to the body methabolism for the detoxification process (Fernandes et al., 2021).
References
Blaise, C., Gagne’, F., Ferard, J. F., Eullaffroy, P. (2008). Ecotoxicity of selected nano-materials to aquatic organisms. Environ Toxicol., 23(5): 591-8. https://doi.org/10.1002/20402.
C. Blaise F. Gagne’ J. F. Ferard P. Eullaffroy 2008Ecotoxicity of selected nano-materials to aquatic organismsEnviron Toxicol23559159810.1002/20402
Braun, A, Sørensen, S. N., Rasmussen, R. F., Hartmann, N. B., Koch, C. B. (2008). Toxicity and bioaccumulation of xenobiotic organic compounds in the presence of aqueous suspensions of aggregates of nano-C(60). Aquat Toxicol., February 18, 86(3): 379-87. https://doi.org/10.1016/j.aquatox.2007.11.019.
A Braun S. N. Sørensen R. F. Rasmussen N. B. Hartmann C. B. Koch 18022008Toxicity and bioaccumulation of xenobiotic organic compounds in the presence of aqueous suspensions of aggregates of nano-C(60)Aquat ToxicolFebruary 1886337938710.1016/j.aquatox.2007.11.019
Boyes, W. K., Thornton, B. L. M., Al-Abed, S. R., Andersen, C. P., Bouchard, D. C., Burgess, R. M., Hubal, E. A. C., Kitchin, K., Reichman, J. R., Rogers, K. R., Ross, J. A., Rygiewicz, P. T., Scheckel, E. G., Thai, S. F., Zepp, R. G., Zucker, R. M. (2017). A comprehensive framework for evaluating the environmental health and safety implications of engineered nanomaterials. Crit Rev Toxicol, 49: 1-44. https://doi.org/10.1080/10408444.2017.1328400.
W. K. Boyes B. L. M. Thornton S. R. Al-Abed C. P. Andersen D. C. Bouchard R. M. Burgess E. A. C. Hubal K. Kitchin J. R. Reichman K. R. Rogers J. A. Ross P. T. Rygiewicz E. G. Scheckel S. F. Thai R. G. Zepp R. M. Zucker 2017A comprehensive framework for evaluating the environmental health and safety implications of engineered nanomaterialsCrit Rev Toxicol4914410.1080/10408444.2017.1328400
Bourdineaud, J. P., Štambuk, A., Šrut, M. et al. (2021). Gold and silver nanoparticles efects to the earthworm Eisenia fetida-the importance of tissue over soil concentrations. Drug Chem Toxicol, 44:12-29. https://doi.org/10.1080/01480545.2019.1567757.
J. P. Bourdineaud A. Štambuk M. Šrut 2021Gold and silver nanoparticles efects to the earthworm Eisenia fetida-the importance of tissue over soil concentrationsDrug Chem Toxicol44122910.1080/01480545.2019.1567757
Buzea, C., Bladino, I. I. P., Roobbie, K. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases, 2(4): MR17 - MR71, 2007. https://doi.org/10.1116/1.2815690.
C. Buzea I. I. P. Bladino K. Roobbie Nanomaterials and nanoparticles: sources and toxicityBiointerphases24MR17 MR71200710.1116/1.2815690
Carbonell, G., Pro, J., Gómez, N. et al. (2009). Sewage sludge applied to agricultural soil: ecotoxicological efects on representative soil. Ecotoxicol Environ Saf, 72: 1309-1319. https://doi.org/10.1016/j.ecoenv.2009.01.007.
G. Carbonell J. Pro N. Gómez 2009Sewage sludge applied to agricultural soil: ecotoxicological efects on representative soilEcotoxicol Environ Saf721309131910.1016/j.ecoenv.2009.01.007
Chen, M., Yin, J., Liang, Y., Yuan, S., Wang, F., Song, M., Wang, H. (2016). Oxidative stress and immunotoxicity induced by graphene oxide in zebrafish. Aquat. Toxicol., 174: 54-60. https://doi.org/10.1016/j.aquatox.2016.02.015.
M. Chen J. Yin Y. Liang S. Yuan F. Wang M. Song H. Wang 2016Oxidative stress and immunotoxicity induced by graphene oxide in zebrafishAquat. Toxicol174546010.1016/j.aquatox.2016.02.015
Chu, S., Majumdar, A. (2012). Opportunities and challenges for a sustainable energy future. Nature, 488. https://doi.org/10.1038/nature11475.
S. Chu A. Majumdar 2012Opportunities and challenges for a sustainable energy futureNature48810.1038/nature11475
Cornelis, G., Hund-Rinke Kerstin, Nico, W. Van den Brink, A. J. Kuhlbusch. (2014). Fate and bioavailability of engineered nanoparticles in soils: a review. Critical Reviews in Environmental Science and Technology, 44: 2720-2764, 2014. https://doi.org/10.1080/10643389.2013.829767.
G. Cornelis Nico Hund-Rinke Kerstin W. Van den Brink A. J. Kuhlbusch 2014Fate and bioavailability of engineered nanoparticles in soils: a reviewCritical Reviews in Environmental Science and Technology4427202764201410.1080/10643389.2013.829767
Courtois, P., Rorat, A., Lemiere, S. et al. (2021). Medium-term efects of Ag supplied directly or via sewage sludge to an agricultural soil on Eisenia fetida earthworm and soil microbial communities. Chemosphere, 269: 128761. https://doi.org/10.1016/j.chemosphere.2020.128761.
P. Courtois A. Rorat S. Lemiere 2021Medium-term efects of Ag supplied directly or via sewage sludge to an agricultural soil on Eisenia fetida earthworm and soil microbial communitiesChemosphere26912876110.1016/j.chemosphere.2020.128761
Dalton, A. B., Collins, S., Munoz, E., Razal, J. M., Ebron, V. H., Ferraris, J. P., Coleman, J. N., Kim, B. G., Baughman, R. H. Super-tough carbon nanotube fibres: these extraordinary composite fibers can be woven into electronic textiles. Nature, v. 423: 703, 2003. https://doi.org/10.1038/423703a.
A. B. Dalton S. Collins E. Munoz J. M. Razal V. H. Ebron J. P. Ferraris J. N. Coleman B. G. Kim R. H. Baughman Super-tough carbon nanotube fibres: these extraordinary composite fibers can be woven into electronic textilesNature423703200310.1038/423703a
Dillon, A. C., Jones, K. M., Bekkedahl, T. A., Kiang, C. H., Bethune, D. S., Heben, M. J. (1997). Storage of hydrogen in single-walled carbon nanotubes. Nature, 386: 377-379, 1997. https://doi.org/10.1038/386377a0.
A. C. Dillon K. M. Jones T. A. Bekkedahl C. H. Kiang D. S. Bethune M. J. Heben 1997Storage of hydrogen in single-walled carbon nanotubesNature386377379199710.1038/386377a0
Du, C., Pan, N. (2006). Supercapacitors using carbon nanotubes films by electrophoretic deposition, J. Power Sources, 160: 1487-1494. https://doi.org/10.1016/jpowsour.2006.02.092.
C. Du N. Pan 2006Supercapacitors using carbon nanotubes films by electrophoretic depositionJ. Power Sources1601487149410.1016/jpowsour.2006.02.092
Federici, G., Shawn, B., Handy, R. (2007). Toxicity of titanium dioxide nanoparticles to rainbow trout (Oncorhynchus mykiss): gill injury, oxidative stress, and other physiological effects. Aquat. Toxicol., 84: 415. https://doi.org/10.1016/j.aquatox.2007.07.009.
G. Federici B. Shawn R. Handy 2007Toxicity of titanium dioxide nanoparticles to rainbow trout (Oncorhynchus mykiss): gill injury, oxidative stress, and other physiological effectsAquat. Toxicol8441510.1016/j.aquatox.2007.07.009
Fernandes, I. F., Utsunomiya, H. S. M., Valverde, B. S. L., Ferraz, J. V. C., Fujiwara, G. H., Gutierrez, D. M., Oliveira, C., Belussi, L. F., Feernandes, M. N., Carvalho, C. S. (2021). Ecotoxicological evaluation of water from the Sorocaba River using na integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802). Chemosphere, 275: 130000. https://doi.org/10.1016/j.chemosphere.2021.130000.
I. F. Fernandes H. S. M. Utsunomiya B. S. L. Valverde J. V. C. Ferraz G. H. Fujiwara D. M. Gutierrez C. Oliveira L. F. Belussi M. N. Feernandes C. S. Carvalho 2021Ecotoxicological evaluation of water from the Sorocaba River using na integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802)Chemosphere27513000010.1016/j.chemosphere.2021.130000
Forbes, V. E., Agatz, A., Ashauer, R. et al. (2021). Mechanistic efect modeling of earthworms in the context of pesticide risk assessment: synthesis of the FORESEE Workshop. Integr Environ Assess Manag, 17: 352-363. https://doi.org/10.1002/ieam.4338.
V. E. Forbes A. Agatz R. Ashauer 2021Mechanistic efect modeling of earthworms in the context of pesticide risk assessment: synthesis of the FORESEE WorkshopIntegr Environ Assess Manag1735236310.1002/ieam.4338
Gall, A., Pascal, A. A., Robert, B. (2015). Vibrational techniques applied to photosynthesis: resonance Raman and fluorescence line-narrowing. Biochimica et Biophysica Acta - Bioenergetics, 1847(1): 12-18. https://doi.org/10.1016/j.bbabio.2014.09.009.
A. Gall A. A. Pascal B. Robert 2015Vibrational techniques applied to photosynthesis: resonance Raman and fluorescence line-narrowingBiochimica et Biophysica Acta - Bioenergetics18471121810.1016/j.bbabio.2014.09.009
Gomes, A. L. S., Balsamo, P. J., Sprogis, A., Ceragiolli, H. J., Silva, T. N., Oliveira, E. C., Cacuro, T. A., Irazusta, S. P. (2018). Avaliação de toxicidade de nanotubos de carbono de parede múltipla (MWCNT). Boletim Técnico da Faculdade de Tecnologia de São Paulo., 45: 16-22.
A. L. S. Gomes P. J. Balsamo A. Sprogis H. J. Ceragiolli T. N. Silva E. C. Oliveira T. A. Cacuro S. P. Irazusta 2018Avaliação de toxicidade de nanotubos de carbono de parede múltipla (MWCNT)Boletim Técnico da Faculdade de Tecnologia de São Paulo451622
Gottschalk, F., Sonderer, T., Scholz, R.W., Nowack, B. (2009). Modeled environmental concentrations of engineered nanomaterials (TiO2, ZnO, Ag, CNT, fullerenes) for different regions. Environ. Sci. Technol. 43: 9216-9222. https://doi.org/10.1021/es9015553.
F. Gottschalk T. Sonderer R.W. Scholz B. Nowack 2009Modeled environmental concentrations of engineered nanomaterials (TiO2, ZnO, Ag, CNT, fullerenes) for different regionsEnviron. Sci. Technol439216922210.1021/es9015553
Gu, X., Liu, Z., Wang, X. et al. (2017). Coupling biological assays with difusive gradients in thin-flms technique to study the biological responses of Eisenia fetida to cadmium in soil. J Hazard Mater, 339: 340-346. https://doi.org/10.1016/j.jhazmat.2017.06.049.
X. Gu Z. Liu X. Wang 2017Coupling biological assays with difusive gradients in thin-flms technique to study the biological responses of Eisenia fetida to cadmium in soilJ Hazard Mater33934034610.1016/j.jhazmat.2017.06.049
Hou,Y, Li, P., Zhou, H., Zhu, X., Chen, H., Lee, J. et al. (2015). Evaluation of β-amyloid peptides fibrillation induced by nanomaterials based on molecular dynamics and surface plasmon resonance. Nanoscience & Nanotechnology, 15: 1110-6. https://doi.org/10.1166/jnn.2015.9069.
Y Hou P. Li H. Zhou X. Zhu H. Chen J. Lee 2015Evaluation of β-amyloid peptides fibrillation induced by nanomaterials based on molecular dynamics and surface plasmon resonanceNanoscience & Nanotechnology151110111610.1166/jnn.2015.9069
Hu, X., Ouyang, S., Mu, L., An, J., Zhou, O. Effects of graphene oxide and oxidized carbon nanotubes on the cellular division, microstructure, uptake, oxidative stress and metabolic profiles. Environ. Sci. Technol, 21: 1-28. 2015 https://doi.org/10.1021/acs.est.5b02102.
X. Hu S. Ouyang L. Mu J. An O. Zhou Effects of graphene oxide and oxidized carbon nanotubes on the cellular division, microstructure, uptake, oxidative stress and metabolic profilesEnviron. Sci. Technol21128201510.1021/acs.est.5b02102
Hu, L., Choi, J. W., Yang, Y., Jeong, S., La Mantia, F., Cui, L-F., Cui, Y. (2009). Highly conductive paper for energy-storage devices. Proc. Natl. Acad. Sci., 106: 21490-21494, 2009. https://doi.org/10.1073/pnas.0908858106.
L. Hu J. W. Choi Y. Yang S. Jeong F. La Mantia L-F. Cui Y. Cui 2009Highly conductive paper for energy-storage devicesProc. Natl. Acad. Sci1062149021494200910.1073/pnas.0908858106
Irazusta, S. P., Ferreira, M. S., Balsamo, P. J., Almeida, L. S., Ceragiolli, H. J. (2021). Toxicity and possibly reduced graphene oxide cellular interaction with Raphidoceles subcapitata: ultrastructural analysis. Research, Society and Development, 10(15): e459101520377. https://doi.org/10.33448/rsd-v10i15.20377.
S. P. Irazusta M. S. Ferreira P. J. Balsamo L. S. Almeida H. J. Ceragiolli 2021Toxicity and possibly reduced graphene oxide cellular interaction with Raphidoceles subcapitata: ultrastructural analysisResearch, Society and Development1015e45910152037710.33448/rsd-v10i15.20377
Irazusta, S. P, Oliveira, E. C., Ceragioli, H. J., Souza, B. F. S, Mendonça, M. C. P., Soares, E. S., Azevedo, J. R., Cruz- Höfling, M. A., Cruz, Z. M. A. (2018). Stress oxidativo e alterações enzimáticas induzidas por nanotubos de carbono de paredes múltiplas (MWCNT) funcionalizados com polietileno glicol no tecido hepático de camundongos. Revinter, 11(1): 05-25. https://doi.org/10.22280/revintervol11ed1.366.
S. P Irazusta E. C. Oliveira H. J. Ceragioli B. F. S Souza M. C. P. Mendonça E. S. Soares J. R. Azevedo M. A. Cruz- Höfling Z. M. A. Cruz 2018Stress oxidativo e alterações enzimáticas induzidas por nanotubos de carbono de paredes múltiplas (MWCNT) funcionalizados com polietileno glicol no tecido hepático de camundongosRevinter111052510.22280/revintervol11ed1.366
Johnston, H. J., Hutchison, G. R., Christensen, F. M., Peters, S., Hankin, S., Aschberger, K., Stone, V. (2010). A critical review of the biological mechanisms underlying the in vivo and in vitro toxicity of carbono nanotubes: The contribution of physico-chemical characteristics. Nanotoxicology, 4(2): 207-246. https://doi.org/10.3109/17435390903569639.
H. J. Johnston G. R. Hutchison F. M. Christensen S. Peters S. Hankin K. Aschberger V. Stone 2010A critical review of the biological mechanisms underlying the in vivo and in vitro toxicity of carbono nanotubes: The contribution of physico-chemical characteristicsNanotoxicology4220724610.3109/17435390903569639
Kahru, A., Dubourguier, H. C. (2010). From ecotoxicology to nanoecotoxicology. Toxicology, 269: 105-19. https://doi.org/10.1016/j.tox.2009.08.016.
A. Kahru H. C. Dubourguier 2010From ecotoxicology to nanoecotoxicologyToxicology26910511910.1016/j.tox.2009.08.016
Keller, A. A., McFerran, S., Lazareva, A., Suh, S. (2013). Global life cycle releases of engineered nanomaterials. J. Nanopart. Res., 15(6): 1-17. https://doi.org/10.1007/s11051-013-1692-4.
A. A. Keller S. McFerran A. Lazareva S. Suh 2013Global life cycle releases of engineered nanomaterialsJ. Nanopart. Res15611710.1007/s11051-013-1692-4
Kim, H. K., Nam, I. W., Yang, B., Yoon, H. N., Lee, H. K., Park, S. (2019). Carbon nanotube (CNT) incorporated cementitious composites for functional construction materials: the state of the art. Composite Structures, 227(1). https://doi.org/10.1016/j.compstruct.2019.111244.
H. K. Kim I. W. Nam B. Yang H. N. Yoon H. K. Lee S. Park 2019Carbon nanotube (CNT) incorporated cementitious composites for functional construction materials: the state of the artComposite Structures227110.1016/j.compstruct.2019.111244
Köktürk, M., Altindag, F., Nas, M., Calimli, M. H. (2021). Ecotoxicological efects of bimetallic PdNi/MWCNT and PdCu/MWCNT nanoparticles onto DNA damage and oxidative stress in earthworms. Biological Trace Element Research, July 27. https://doi.org/10.1007/s12011-021-02821-z.
M. Köktürk F. Altindag M. Nas M. H. Calimli 27072021Ecotoxicological efects of bimetallic PdNi/MWCNT and PdCu/MWCNT nanoparticles onto DNA damage and oxidative stress in earthwormsBiological Trace Element ResearchJuly 2710.1007/s12011-021-02821-z
Kwok, K. W., Leung, K. M., Flahaut, E., Cheng, J., Cheng, S. H. (2010). Chronic toxicity of double-walled carbon nanotubes to three marine organisms: influence of different dispersion methods. Nanomedicine, 5(6): 951-961, 2010. https://doi.org/10.2217/nnm.10.59.
K. W. Kwok K. M. Leung E. Flahaut J. Cheng S. H. Cheng 2010Chronic toxicity of double-walled carbon nanotubes to three marine organisms: influence of different dispersion methodsNanomedicine56951961201010.2217/nnm.10.59
Li, S., Irin, F., Atore, F. O. et al. (2013). Determination of multi-walled carbon nanotube bioaccumulation in earthworms measured by a microwave-based detection technique. Sci Total Environ, 445-446: 9-13. https://doi.org/10.1016/j.scitotenv.2012.12.037.
S. Li F. Irin F. O. Atore 2013Determination of multi-walled carbon nanotube bioaccumulation in earthworms measured by a microwave-based detection techniqueSci Total Environ445-44691310.1016/j.scitotenv.2012.12.037
Lobo, A. O., Ramos, S. C., Antunes, E. F., Marciano, F. R., Trava-Airoldi, V. J., Corat, E. J. (2012). Fast functionalization of vertically aligned multi-walled carbon nanotubes using oxygen plasma. Materials Letters, 70: 89-93. https://doi.org/10.1016/j.matlet.2011.11.071.
A. O. Lobo S. C. Ramos E. F. Antunes F. R. Marciano V. J. Trava-Airoldi E. J. Corat 2012Fast functionalization of vertically aligned multi-walled carbon nanotubes using oxygen plasmaMaterials Letters70899310.1016/j.matlet.2011.11.071
Long, Z. F., Ji, J., Yang, K., Lin, D. H., Wu, F. C. (2012). Systematic and quantitative investigation of the mechanism of carbon nanotubes’ toxicity toward algae. Environ. Sci. Technol., 46: 8458-8466. https://doi.org/10.1021/es301802g.
Z. F. Long J. Ji K. Yang D. H. Lin F. C. Wu 2012Systematic and quantitative investigation of the mechanism of carbon nanotubes’ toxicity toward algaeEnviron. Sci. Technol468458846610.1021/es301802g
Lovern, S. B., Stricler, J. R., Klaper, R. (2007). Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano-C60, and C60HxC70Hx). Environ. Sci. Technol., 41: 4465-72. https://doi.org/10.1021/es062146p.
S. B. Lovern J. R. Stricler R. Klaper 2007Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano-C60, and C60HxC70Hx)Environ. Sci. Technol414465447210.1021/es062146p
Lu, T., Zhang, Q., Zhang, Z., Hu, B., Chen, J., Qian, J. C. H. (2021). Pollutant toxicology with respect to microalgae and cyanobacteria. Journal of Environmental Sciences, 99: 175-186. https://doi.org/10.1016/j.jes.2020.06.033.
T. Lu Q. Zhang Z. Zhang B. Hu J. Chen J. C. H. Qian 2021Pollutant toxicology with respect to microalgae and cyanobacteriaJournal of Environmental Sciences9917518610.1016/j.jes.2020.06.033
Magdolenova, Z. et al. (2014). Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology, 8(3): 233-278. https://doi.org/10.3109/17435390.2013.773464.
Z. Magdolenova 2014Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticlesNanotoxicology8323327810.3109/17435390.2013.773464
Mahapatra, I. et al. (2015). Probabilistic modelling of prospective environmental concentrations of gold nanoparticles from medical applications as a basis for risk assessment. Journal of Nanobiotechnology, 13: 1-14. https://doi.org/10.1186/s12951-015-0150-0.
I. Mahapatra 2015Probabilistic modelling of prospective environmental concentrations of gold nanoparticles from medical applications as a basis for risk assessmentJournal of Nanobiotechnology1311410.1186/s12951-015-0150-0
Mauter, M. S., Elimelech, M. (2008). Environmental applications of carbon-based nanomaterials. Environ. Sci. Technol., 42: 5843-5859. https://doi.org/10.1021/es8006904.
M. S. Mauter M. Elimelech 2008Environmental applications of carbon-based nanomaterialsEnviron. Sci. Technol425843585910.1021/es8006904
Mitrano, D. M., Motellier, S., Clavaguera, S., Nowack, B. (2015). Review of nanomaterial aging and transformations through the life cycle of nano-enhanced products. Environ Int., 77: 132-147. https://doi.org/10.1016/j.envint.2015.01.013.
D. M. Mitrano S. Motellier S. Clavaguera B. Nowack 2015Review of nanomaterial aging and transformations through the life cycle of nano-enhanced productsEnviron Int.7713214710.1016/j.envint.2015.01.013
Neal, A. L. (2008). What can be inferred from bacterium-nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles? Ecotoxicology, 17: 362- 371. https://doi.org/10.1007/s10646-008-0217-x.
A. L. Neal 2008What can be inferred from bacterium-nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles?Ecotoxicology17362 37110.1007/s10646-008-0217-x
Nogueira, P. F. M., Nakabayashi, D., Zucolotto, V. (2015). The effects of graphene oxide on green algae Raphidocelis subcapitata. Aquatic Toxicology, 166: 29-35. https://doi.org/10.1016/j.aquatox.2015.07.001.
P. F. M. Nogueira D. Nakabayashi V. Zucolotto 2015The effects of graphene oxide on green algae Raphidocelis subcapitataAquatic Toxicology166293510.1016/j.aquatox.2015.07.001
Nowack, B., James F. Ranville, Stephen Diamond, Julian A. Gallego-Urrea, Chris Metcalfe, Jerome Rose, Nina Horne, Albert A. Koelmans, Stephen J. Klaine. (2012). Potential scenarios for nanomaterial release and subsequent alteration in the environment. Environmental Toxicology and Chemistry, 31(1): 50-59. https://doi.org/10.1002/etc.726.
B. Nowack James F. Ranville Stephen Diamond Julian A. Gallego-Urrea Chris Metcalfe Jerome Rose Nina Horne Albert A. Koelmans Stephen J. Klaine 2012Potential scenarios for nanomaterial release and subsequent alteration in the environmentEnvironmental Toxicology and Chemistry311505910.1002/etc.726
Oberdörster, G., Oberdörster, E., Oberdörster, J. (2005). Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect., July, 113(7): 823-39, 2005. https://doi.org/10.1289/ehp.7339.
G. Oberdörster E. Oberdörster J. Oberdörster 072005Nanotoxicology: an emerging discipline evolving from studies of ultrafine particlesEnviron Health PerspectJuly1137823839200510.1289/ehp.7339
Pakarinen, K., Petersen, E. J., Alvila, L., Waissi-leinonen, G. C., Akkanen, J., Leppänen, M. T., Kukkonen, J. V. K. (2013). A screening study on the fate of fullerenes (nC60) and their toxic implications in natural freshwaters. Environ. Toxicol. Chem. 32: 1224-1232, 2013. https://doi.org/10.1002/etc.2175.
K. Pakarinen E. J. Petersen L. Alvila G. C. Waissi-leinonen J. Akkanen M. T. Leppänen J. V. K. Kukkonen 2013A screening study on the fate of fullerenes (nC60) and their toxic implications in natural freshwatersEnviron. Toxicol. Chem3212241232201310.1002/etc.2175
Parab, N. D. T., Tomar, V. (2012). Raman spectroscopy of algae: a review. Journal of Nanomedicine and Nanotechnology, 3(2). https://doi.org/10.4172/2157-7439.1000131.
N. D. T. Parab V. Tomar 2012Raman spectroscopy of algae: a reviewJournal of Nanomedicine and Nanotechnology3210.4172/2157-7439.1000131
Paschoalino, M. P., Marcone, G. P. S., Jardim, W. F. (2010). Os nanomateriais e a questão ambiental. Quím. Nova. 33b(2): 421-430. https://doi.org/10.1590/s0100-40422010000200033.
M. P. Paschoalino G. P. S. Marcone W. F. Jardim 2010Os nanomateriais e a questão ambientalQuím. Nova33b242143010.1590/s0100-40422010000200033
Patel, A., Tiwari, S., Parihar, P., Singh, R., Prasad, S. M. (2019). Carbon nanotubes as plant growth regulators: impacts on growth, reproductive system, and soil microbial community. In Tripathi, D. K., Ahmad, P., Sharma, S., Chauhan, D. K., Dubey, N. K. (eds.). Nanomaterials in plants, algae and microorganisms. Cambridge: Academic Press, 23-42. https://doi.org/10.1016/b978-0-12-811488-9.00002-0.
A. Patel S. Tiwari P. Parihar R. Singh S. M. Prasad 2019Carbon nanotubes as plant growth regulators: impacts on growth, reproductive system, and soil microbial community D. K. Tripathi P. Ahmad S. Sharma D. K. Chauhan N. K. Dubey Nanomaterials in plants, algae and microorganismsCambridgeAcademic Press234210.1016/b978-0-12-811488-9.00002-0
Petersen, E. J., Huang, Q., Weber, W. J. (2010). Relevance of octanol water distribution measurements to the potential ecological uptake of multi-walled carbon nanotubes. Environ Toxicol Chem., 29: 1106-1112. https://doi.org/10.1002/etc.149.
E. J. Petersen Q. Huang W. J. Weber 2010Relevance of octanol water distribution measurements to the potential ecological uptake of multi-walled carbon nanotubesEnviron Toxicol Chem291106111210.1002/etc.149
Petersen, E. J., Henry, E. B. (2012). Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: review. Environ. Toxicol. and Chem., 31(1): 60-72.
E. J. Petersen E. B. Henry 2012Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: reviewEnviron. Toxicol. and Chem3116072
Ramrakhiani, M. (2012). Nanostructures and their applications. Recent Research in Science and Technology, 4(8): 14-19, 2012. https://doi.org/10.1002/etc.710.
M. Ramrakhiani 2012Nanostructures and their applicationsRecent Research in Science and Technology481419201210.1002/etc.710
Reynolds, A., Giltrap, D. M., Chambers, P. G. (2021). Acute growth inhibition & toxicity analysis of nano-polystyrene spheres on Raphidocelis subcapitata. Ecotoxicology and Environmental Safety, 207(April): 111153. https://doi.org/10.1016/j.ecoenv.2020.111153.
A. Reynolds D. M. Giltrap P. G. Chambers 042021Acute growth inhibition & toxicity analysis of nano-polystyrene spheres on Raphidocelis subcapitataEcotoxicology and Environmental Safety207April11115310.1016/j.ecoenv.2020.111153
Ren, W., Ren, G., Teng, Y., Li, Z., Li, L. (2015). Time-dependent effect of graphene on the structure, abundance, and function of the soil bacterial community. J. Hazard Mater., 297: 286-294. https://doi.org/10.1016/j.jhazmat.2015.05.017.
W. Ren G. Ren Y. Teng Z. Li L. Li 2015Time-dependent effect of graphene on the structure, abundance, and function of the soil bacterial communityJ. Hazard Mater29728629410.1016/j.jhazmat.2015.05.017
Saxena, F. P., Sangela, V., Ranjan, S., Dutta, V., Dasgupta, N., Phulwaria, M., Rathore, D. S. (2020). Aquatic nanotoxicology: impact of carbon nanomaterials on algal. Energ. Ecol. Environ., 5: 240-252. https://doi.org/10.1007/s40974-020-00151-9.
F. P. Saxena V. Sangela S. Ranjan V. Dutta N. Dasgupta M. Phulwaria D. S. Rathore 2020Aquatic nanotoxicology: impact of carbon nanomaterials on algalEnerg. Ecol. Environ524025210.1007/s40974-020-00151-9
Schwab, F., Bucheli, T. D., Lukhele, L. P., Magrez, A., Nowack, B. , Sigg, L., Knauer, K. (2011). Are carbono nanotube effects on green algae caused by shading and agglomeration? Environ. Sci. Technol., 45: 6136-6144. https://doi.org/10.1021/es200506b.
F. Schwab T. D. Bucheli L. P. Lukhele A. Magrez B. Nowack L. Sigg K. Knauer 2011Are carbono nanotube effects on green algae caused by shading and agglomeration?Environ. Sci. Technol456136614410.1021/es200506b
Singh, N. P. et al. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res, 175: 184-91. https://doi.org/10.1016/0014-4827(88)90265-0.
N. P. Singh 1988A simple technique for quantitation of low levels of DNA damage in individual cellsExp. Cell. Res17518419110.1016/0014-4827(88)90265-0
Snow, E. S., Perkins, F. K., Houser, E. J., Badescu, S. C., Reinecke, T. L. (2005). Chemical detection with a single-walled carbon nanotube capacitor. Science, 307: 1942-1945. https://doi.org/10.1126/science.1109128.
E. S. Snow F. K. Perkins E. J. Houser S. C. Badescu T. L. Reinecke 2005Chemical detection with a single-walled carbon nanotube capacitorScience3071942194510.1126/science.1109128
Sohn, E. K., Chung, Y. S ., Johari, S. A., Kim, T. G., Kim, J. K., Lee, J. H., Lee, Y. H., Kang, S. W., Yu, J. (2015). Acute toxicity comparison of single-walled carbon nanotubes in various freshwater organisms. BioMed Research International, article ID 323090, 7. https://doi.org/10.1155/2015/323090.
E. K. Sohn Y. S . Chung S. A. Johari T. G. Kim J. K. Kim J. H. Lee Y. H. Lee S. W. Kang J. Yu 2015Acute toxicity comparison of single-walled carbon nanotubes in various freshwater organismsBioMed Research International323090710.1155/2015/323090
Sun, W., Meng, Z., Li, R. et al. (2020). Joint efects of microplastic and dufulin on bioaccumulation, oxidative stress and metabolic profle of the earthworm (Eisenia fetida). Chemosphere, 263: 128171. https://doi.org/10.1016/j.chemosphere.2020.128171.
W. Sun Z. Meng R. Li 2020Joint efects of microplastic and dufulin on bioaccumulation, oxidative stress and metabolic profle of the earthworm (Eisenia fetida)Chemosphere26312817110.1016/j.chemosphere.2020.128171
Tong, Z., Bischoff, M., Nies, L., Applegate, B., Turco, R. F. (2007). Impact of fullerene (C60) on a soil microbial community. Environ. Sci. Technol., 41: 2985-92. https://doi.org/10.1021/es061953l.
Z. Tong M. Bischoff L. Nies B. Applegate R. F. Turco 2007Impact of fullerene (C60) on a soil microbial communityEnviron. Sci. Technol412985299210.1021/es061953l
Tourinho, P. S. et al. (2012). Metal based nanoparticles in soil: fate, behavior, and effects on soil invertebrates. Environmental Toxicology and Chemistry, 31(8): 1679-1692. https://doi.org/10.1002/etc.1880.
P. S. Tourinho 2012Metal based nanoparticles in soil: fate, behavior, and effects on soil invertebratesEnvironmental Toxicology and Chemistry3181679169210.1002/etc.1880
Unrine, J. M., Tsyusko, O. V., Hunyadi, S. E. et al. (2010). Efects of particle size on chemical speciation and bioavailability of copper to earthworms (Eisenia fetida) exposed to copper nanoparticles. J. Environ Qual., 39: 1942-1953. https://doi.org/10.2134/jeq2009.0387.
J. M. Unrine O. V. Tsyusko S. E. Hunyadi 2010Efects of particle size on chemical speciation and bioavailability of copper to earthworms (Eisenia fetida) exposed to copper nanoparticlesJ. Environ Qual391942195310.2134/jeq2009.0387
Valant, J., Drobne, D., Novak, S. (2012). Effect of ingested titanium dioxide nanoparticles on the digestive gland cell membrane of terrestrial isopods. Chemosphere, 87(1): 19-25. https://doi.org/10.1016/j.chemosphere.2011.11.047.
J. Valant D. Drobne S. Novak 2012Effect of ingested titanium dioxide nanoparticles on the digestive gland cell membrane of terrestrial isopodsChemosphere871192510.1016/j.chemosphere.2011.11.047
Van der Zande, M., Junker, R., Walboomers, X. F., Jansen, J. A. (2010). Carbon nanotubes in animal models: a systematic review on toxic potential. Tissue Eng., Part B, 17(1): 57-69. https://doi.org/10.1089/tem.TEB.2010.0472.
M. Van der Zande R. Junker X. F. Walboomers J. A. Jansen 2010Carbon nanotubes in animal models: a systematic review on toxic potentialTissue Eng.B171576910.1089/tem.TEB.2010.0472
Vicentini, R., Costa, H. L., Nunes, W., Vilas Boas, O., Soares, D. M., Alves, T. A., Real, C., Bueno, C., Peterlevitz, A. C., Zanin, H. (2018). Direct growth of mesoporous carbon on aluminum foil for supercapacitors devices. Journal of Materials Science: Materials in Electronics, 29(6): 1-10. https://doi.org/10.1007/s10854-018-9121-1.
R. Vicentini H. L. Costa W. Nunes O. Vilas Boas D. M. Soares T. A. Alves C. Real C. Bueno A. C. Peterlevitz H. Zanin 2018Direct growth of mesoporous carbon on aluminum foil for supercapacitors devicesJournal of Materials Science: Materials in Electronics29611010.1007/s10854-018-9121-1
Vicentini, R., Nunes, W. G., Costa, L. H., Da Silva, L. M., Pascona, A., Jackson, P., Doubekc, G., Zanin, H. (2019). Highly stable nickel-aluminum alloy current collectors and highly defective multi-walled carbon nanotubes active material for neutral aqueous-based electrochemical capacitors. Journal of Energy Storage, 23: 116-127. https://doi.org/10.1016/j.est.2019.01.013.
R. Vicentini W. G. Nunes L. H. Costa L. M. Da Silva A. Pascona P. Jackson G. Doubekc H. Zanin 2019Highly stable nickel-aluminum alloy current collectors and highly defective multi-walled carbon nanotubes active material for neutral aqueous-based electrochemical capacitorsJournal of Energy Storage2311612710.1016/j.est.2019.01.013
Waissi, G. C., Bold, S., Pakarinen, K., Akkanen, J., Leppanen, M. T., Petersen, E. J., J. V. K. Kukkonen. (2017). Chironomus riparius exposure to fullerene-contaminated sediment results in oxidative stress and may impact life cycle parameters. J. Hazard Mater, January, 15(322)(Pt A): 301-309. https://doi.org/10.1016/jhazmat.2016.04.015.
G. C. Waissi S. Bold K. Pakarinen J. Akkanen M. T. Leppanen E. J. Petersen J. V. K. Kukkonen 012017Chironomus riparius exposure to fullerene-contaminated sediment results in oxidative stress and may impact life cycle parametersJ. Hazard MaterJanuary15322A30130910.1016/jhazmat.2016.04.015
Wick, P., Manser, P., Limbach, L. K., Dettlaff-Weglikowska, U., Krumeich, F., Roth, S., et al. (2007). The degree and kind of agglomeration affect carbon nanotube cytotoxicity. Toxicology Letters., 168: 121-131. https://doi.org/10.1016/j.toxlet.2006.08.019.
P. Wick P. Manser L. K. Limbach U. Dettlaff-Weglikowska F. Krumeich S. Roth 2007The degree and kind of agglomeration affect carbon nanotube cytotoxicityToxicology Letters16812113110.1016/j.toxlet.2006.08.019
Wang, H., Shao, B., Yu, H. et al. (2019). Neuroprotective role of hyperforin on aluminum maltolate-induced oxidative damage and apoptosis in PC12 cells and SH-SY5Y cells. Chem Biol Interact, 299: 15-26. https://doi.org/10.1016/j.cbi.2018.11.016.
H. Wang B. Shao H. Yu 2019Neuroprotective role of hyperforin on aluminum maltolate-induced oxidative damage and apoptosis in PC12 cells and SH-SY5Y cellsChem Biol Interact299152610.1016/j.cbi.2018.11.016
Xiao, L., Li, M., Hu, J., Dai, J. et al. (2020). Assessment of earthworm activity on Cu, Cd, Pb and Zn bioavailability in contaminated soils using biota to soil accumulation factor and DTPA extraction. Ecotoxicol Environ Saf, 195: 110513. https://doi.org/10.1016/j.ecoenv.2020.110513.
L. Xiao M. Li J. Hu J. Dai 2020Assessment of earthworm activity on Cu, Cd, Pb and Zn bioavailability in contaminated soils using biota to soil accumulation factor and DTPA extractionEcotoxicol Environ Saf19511051310.1016/j.ecoenv.2020.110513
Zhang, B.T., Zheng, X., Li, H. F., Lin, J. M. (2013). Application of carbon-based nanomaterials in sample preparation: a review. Anal. Chim. Acta, 784: 1-17. https://doi.org/10.1016/j.aca.2013.03.05410.
B.T. Zhang X. Zheng H. F. Li J. M. Lin 2013Application of carbon-based nanomaterials in sample preparation: a reviewAnal. Chim. Acta78411710.1016/j.aca.2013.03.05410
Zhang, L., Lei, C., Chen, J., Yang, Kun, Zhu, L., Lin, D. (2015). Effect of natural and synthetic surface coatings on the toxicity of multi-walled carbon nanotubes toward green algae. Carbon, 83: 198-207. https://doi.org/10.1016/j.carbon.2014.11.050.
L. Zhang C. Lei J. Chen Kun Yang L. Zhu D. Lin 2015Effect of natural and synthetic surface coatings on the toxicity of multi-walled carbon nanotubes toward green algaeCarbon8319820710.1016/j.carbon.2014.11.050
Zhang, M., Wang, H., Song, Y., Huang, H., Shao, M., Liu, Y., Kang, Z. (2018). Pristine carbon dots boost the growth of Chlorella vulgaris by enhancing photosynthesis. ACS Appl Bio Mater, 1(3): 894-902. https://doi.org/10.1021/acsabm.8b00319.
M. Zhang H. Wang Y. Song H. Huang M. Shao Y. Liu Z. Kang 2018Pristine carbon dots boost the growth of Chlorella vulgaris by enhancing photosynthesisACS Appl Bio Mater1389490210.1021/acsabm.8b00319
Zhang, C., Chen, X., Tan, L., Wang, J. (2018). Combined toxicities of copper nanoparticles with carbon nanotubes on marine microalgae Skeletonema costatum. Environ Sci Pollut Res, 25(13): 13127-13133. https://doi.org/10.1007/s11356-018-1580-7.
C. Zhang X. Chen L. Tan J. Wang 2018Combined toxicities of copper nanoparticles with carbon nanotubes on marine microalgae Skeletonema costatumEnviron Sci Pollut Res2513131271313310.1007/s11356-018-1580-7
Zhao, C-M., Wang, W-X. (2010). Biokinetic uptake and efflux of silver nanoparticles in daphnia magna. Environ. Sci. Technol., 44: 7699-7704. https://doi.org/10.1021/es101484s.
C-M. Zhao W-X. Wang 2010Biokinetic uptake and efflux of silver nanoparticles in daphnia magnaEnviron. Sci. Technol447699770410.1021/es101484s
Zhao, X. C., Liu, R. T. (2012). Recent progress and perspectives on the toxicity of carbon nanotubes at organism, organ, cell, and biomacromolecule levels. Environ. Int., 40: 244-255.
X. C. Zhao R. T. Liu 2012Recent progress and perspectives on the toxicity of carbon nanotubes at organism, organ, cell, and biomacromolecule levelsEnviron. Int40244255
Zhao, Y., Liu, Y., Zhang, X, Liao, W. (2021). Environmental transformation of graphene oxide in the aquatic environment. Chemosphere, e(262): 127885. https://doi.org/10.1016/j.envint.2011.12.003.
Y. Zhao Y. Liu X Zhang W. Liao 2021Environmental transformation of graphene oxide in the aquatic environmentChemospheree26212788510.1016/j.envint.2011.12.003